Background: High-quality HIV rapid testing is essential for accurate diagnosis, timely treatment initiation, and progress toward HIV epidemic control. Despite the national scale-up of HIV testing in Cameroon, variations in quality management practices across facilities may compromise diagnostic reliability. This study examined disparities in the quality of HIV rapid testing by donor support, organizational affiliation, and geographic location.
Methods: A cross-sectional evaluation was conducted among 99 HIV rapid testing laboratories in the South Region of Cameroon from 2022 to 2024 using the World Health Organization Stepwise Process for Improving the Quality of HIV Rapid Testing (SPI-RRT). Seven core quality elements were assessed. Facilities were stratified by donor support and organizational affiliation, and performance was classified into readiness Levels 0-4. Descriptive and comparative analyses were performed. Key findings are presented in Table 1 (donor support), Table 2 (organizational affiliation), and Figure 1 (district-level readiness).
Results: PEPFAR-supported laboratories achieved a higher mean overall SPI-RRT score (59 ± 4) than non-PEPFAR-supported facilities (14 ± 6) (Table 1). Only 12% of laboratories reached Level 3 or 4 readiness for certification. Community-based organizations recorded the highest mean score (50 ± 3), while government (28 ± 21), faith-based (25 ± 23), and private facilities (25 ± 17) showed lower and variable performance (Table 2). District-level analysis indicated higher readiness in Sangmelima and Kribi, whereas most rural districts remained at Level 0 or 1 (Figure 1).
Conclusions: HIV rapid testing quality in the South Region of Cameroon is strongly influenced by donor support, organizational affiliation, and district context. Strengthening mentorship, supervision, and continuous quality improvement is critical to improving accreditation readiness and ensuring reliable HIV diagnosis, with implications for HIV prevention, treatment, and care.
Keywords: HIV rapid testing; quality assurance; SPI-RRT
